Laboratory of Protein Hormone Chemistry

Head of the Laboratory: Oleg V. Sviridov
D. Sc. (chemistry)
Laureate of State Prize of Republic of Belarus

 

phone: +375 (17) 369-87-51, 396-47-51
e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.

 

 

 

 

 

 

 

 

 

 

 


About the Laboratory

Established in 1979.

From 1979 to 2000 the laboratory was headed by Oleg A. Strel'chyonok, D.Sc., professor, academician of NASB.

Personnel: 14 researchers including 1 D.Sc. and 4 Ph.D.

 


Research directions

  • Bioorganic chemistry of hormone binding and hormone producing proteins, autoantigens and autoantibodies
  • Mechanisms and chemical and structural aspects of ligand-protein binding
  • Design and techniques for immunochemical protein and hormone diagnostics and food and feed control for antibiotics and mycotoxins content.

Main achievements and developments

  • Isolation and identification of one and the same linear alpha-(1-6)-D-glucan with a molecular mass of about 5 kDa from probiotic microorganism Bifidobacterium bifidum BIM B-733D by immunoaffinity chromatography on three different sorbents with immobilized human antibodies toward thyroid peroxidase, thyroglobulin and gliadins. Also, it was shown that the glucan interacts selectively with human autoantibodies to thyroid peroxidase, thyroglobulin, tissue transglutaminase and human anti-gliadin antibodies. Based on the data obtained, it was hypothesized that the uncovered unique glucan interacts selectively with the Fab-fragment of all human autoantibodies and distinguishes them from antibodies to exogenous antigens
  • Development of techniques and reagent kits for various kinds of immunoassays (radio, enzyme, time-resolved fluorimetric, and chromatographic)  for steroid, thyroid and protein hormones, protein markers, antibiotics and mycotoxins as tools for medical diagnostics and food and feed safety control. Immunoassay kits are manufactured at the unitary enterprise "Pilot Production of the Institute of Bioorganic Chemistry National Academy of Sciences of Belarus" as well as serially produced at the laboratory's production technological section under the PRODOSCRIN® trademark, and are supplied to customers
  • It was shown that steroid hormone complexes with human plasma glycoproteins including their molecular variants having specific carbohydrate structures (transcortin, SBG) interact with receptors in the plasma membranes of specific human tissues. Thyroid hormones are associated with the multicomponent system of human plasma thyroxine-binding proteins (TBG and its molecular variants, apolipoprotein AI, immunoglobulin M, albumin), whose components differ in affinity for these ligands and have specific functions in the hormonal action and metabolism of iodothyronines
  • Elucidation of the unknown earlier ability of human alpha-1-microglobulin - a protein of lipocalin family - to bind thyroid and steroid
    hormones, and neonicotinoid pesticides predominantly by hydrophobic interactions.

 


Selected publications

  1. Kiseleva E.P., Mikhailopulo K.I., Zdorovenko E.L., Knirel Y.A., Novik G.I. Linear α-(1→6)-D-glucan from Bifidobacterium bifidum BIM B-733D is low molecular mass biopolymer with unique immunochemical properties // Carbohydr. Res. - 2018, V.466, P. 39-50, DOI: 10.1016/ j.carres.2017.12.008.
  2. Serchenya T.S., Shcharbin D., Shyrochyna I., Sviridov O., Terekhova M., Dzmitruk V., Abashkin V., Apartsin E., Mignani S., Majoral J.-P., Ionov M., Bryszewska M. Immunoreactivity changes of human serum albumin and alpha-1-microglobulin induced by their interaction with dendrimers. // Colloids and Surfaces B: Biointerfaces. - 2019, V.179, P.226-232.
  3. Serchenya T.S., Sviridov O.V. Specific binding of insecticide substances of the neonicotinoid group to albumin and human alpha-1-microglobulin. // Doklady Nat. Acad. Sci. Bel. (Rus.). - 2013, V.57, no. 3, P.89-94.
  4. Navakouski M.E., Vashkevich I.I., Sviridov O.V. Interaction of thriiodothyronine-biotin conjugate with binding proteins in immunoassay systems. // Rus. J. Bioorg. Chem. - 2012, V.38, P.392-399.
  5. Strel'chyonok O.A., Avvakumov G.V. Specific steroid-binding glycoproteins of human blood plasma: novel data on their structure and function.//J. Steroid Biochem. - 1990, V.35, no. 5, P.519-34.

 

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