Laboratory of Molecular Diagnostics and Biotechnology

 

Head of the Laboratory: Gennady V. Sergeev
Ph.D. (chemistry)

 

 


тел. +375 (17) 369-87-77

e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.

 

 

 

 

 


About the Laboratory

Established in 2009 (from 2009 to 2017 the laboratory was headed by Audrey A. Gilep, Ph.D.).

Personnel: 21 researchers including 7 Ph.D.


Research directions

  • Structure-function studies of cytochromes P450 of humans and pathogenic organisms
  • Developments in the area of recombinant antibodies for human disease treatment
  • Study of enzymes of pathogenic fungi (causative agents of mycoses in humans), search for inhibitors of their activity, identification of resistant to antifungal drugs strains
  • Development of PCR kits for diagnostics of infectious diseases of humans and animals
  • Elaboration of sample pretreatment methods: kits for isolating DNA and RNA from biological fluids, tissues of animal and vegetable origin, kits for isolating plasmid DNA and for purifying DNA from agarose gel
  • Molecular diagnostics in high performance sport
  • Development of methods for estimation of the level of drug metabolism for personalized medicine (psychiatry, cardiology, and oncology).

Main achievements and developments

  • Determination of the molecular mechanism of resistance of the pathogen Mycobacterium tuberculosis in order to create a new generation of anti-tuberculosis drugs.
  • Highly purified human recombinant cytochrome P450 preparations: CYP19, CYP11B2, CYP17, CYP51, CYP2C9.1,
    CYP2C9.2, CYP2C9.3, CYP2C19.1A, CYP2C19.1B, CYP2C19.9 and CYP2C19.13.
  • A reagent kit DNA-VK for isolating the total DNA from samples using inorganic sorbent.

Selected Publications

  1. Masamrekha R., Kuzikov A., Veselovsky A., Toropygin I., Shkel T., Strushkevich N., Gilep A., Usanov S., Archakov A., Shumyantseva V. Interaction of 17α-hydroxylase, 17(20)-lyase (CYP17A1) inhibitors - abiraterone and galeterone — with human sterol 14α-demethylase (CYP51A1). //Journal of Inorganic Biochemistry. - 2018, V.186, P.24-33.
  2. Haidukevich I.V., Sushko T.A.. Tumilovich A.M., Grabovec I.P., Usanov S.A., Gilep A.A. Different inhibitory effects of azole-containing drugs and pesticides on CYP2C9 polymorphic forms: an in vitro study. // Toxicology in Vitro. - 2018, V.50, P.249-256.
  3. Dormeshkin D., Gilep A., Sergeev G., Usanov S. Development of CYB5-fusion monitoring system for efficient periplasmic expression of multimeric proteins in Escherichia coli. // Protein Expr. Purif - 2016, V.128, P 60-66.
  4. Svirid A.V., Ershov P.V., Yablokov E.O., Kaluzhskiy L.A., Mezentsev Y.V., Florinskaya A.V., Sushko T.A., Strushkevich N.V., Gilep A.A., Usanov S.A., Medvedev A.E., Ivanov A.S. Direct molecular fishing of new protein partners for human thromboxane synthase. // Acta Naturae. - 2017, V.9 (4), P.92-100.
  5. Davydov R., Gilep A.A., Strushkevich N.V., Usanov S.A., Hoffman B.M Compound I is the reactive intermediate in the first monooxygenation step during conversion of cholesterol to pregnenolone by cytochrome P450scc: EPR/ENDOR/cryoreduction/annealing studies. // J Am Chem Soc. - 2012, V.134 (41), P.17149-I7156.
  6. Sergeev G., Ivanov A.S., Medvedev A., Ershov P., Molnar A., Mezentsev Yu., Yablokov E., Kaluzhsky L., Gnedenko O., Buneeva O., Haidukevich I., Lushchyk A., Yantsevich A., Medvedeva M., Kozin S., Popov I., Novikova S., Zgoda V., Gilep A., Usanov S., Lisitsa A., Archakov A. Protein interactomics based on direct molecular fishing on paramagnetic particles: practical realization and further SPR validation. // Proteomics. - 2014, V.14, P.2261-2274.

 

 

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