Laboratory of Lipid Chemistry

Acting Head of laboratory - Mikhalchuk A.L., Ph.D.

 

 

 

tel. +375 (17) 3699131

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About laboratory

The laboratory was founded in 2001 after the restructuring of the laboratory of bioorganic chemistry. From that moment till 2018 the laboratory has been headed by prof. Mikhail Kisel.

The research team of the laboratory consists of 11 scientists (including 1 Sc.D. and 6 Ph.D.) and 2 post graduate students.

The researchers of the laboratory defended 4 Ph.D. theses

  1. Vladimir Dolgopalets (2001)
  2. Igor Trostyanko (2009)
  3. Olga Sharko (2010)
  4. Dzmitry Arsenau (2010)

Research Areas

  • Chemical and enzymatic synthesis of physiologically active lipids and related compounds;
  • Study of enzymatic and free-radical reactions mechanisms leading to the signal lipids formation and the addressed regulation of such reactions by lipid effectors;
  • Construction of liposomal drug-delivery systems;
  • Synthesis and investigation of lipoid compounds for plant protection.

Applied Research

  1. Technological process for 5-aminolevulinic acid hydrochloride which is the chemical substance for "Alamin" – a preparation for diagnostics and therapy of bladder cancer.
  2. Technological process for 5-aminolevulinic acid hexyl ester which is the effective regulator of plant growth.
  3. Preparative form of systemic fungicide "Baifucide" (active substance difenoconazole) for plant protection against fungal disease.
  4. Original synthesis of 16-doxyl-stearic acid which is used for diagnostics and therapy monitoring of malignant tumors.
  5. Fluorescent and chromogenic lipid substrates for assaying lipolytic enzymes.

Selected Publications

  1. D.V. Arsenov, I.G. Dad'kov, M.A. Kisel, B.B. Kuz'mitskii, O. A. Strelchenok. Synthesis of N-(all-trans-Retinoyl)doxorubicin and study of the antitumor activity of its complex with blood serum proteins. Pharmaceutical Chemistry Journal, 2001, 35(4), 10-13. DOI: 10.1023/A:1010419726510
  2. S.G. Spivak, M.A. Kisel, G.A. Yakovleva. Boosting the immune system of Solanaceous plants by lysophosphatidylcholine. Russian Journal of Plant Physiology, 2003, 50(3), 293-296. DOI: 10.1023/A:1023801732305
  3. Lodyato V.I., Yurkova I.L., Sorokin V.L., Shadyro O.I., Dolgopalets V.I., Kisel M.A. Novel (3,5-Di-tert-2-hydroxy-phenylcarbamoyl)-alkanoic acids as potent antioxidants. Bioorg. & Med. Chem. Lett., 2004, 14, 4253-4256. DOI: 10.1016/j.bmcl.2004.06.003
  4. Shadyro O.I., Yurkova I.L., Kisel M.A., Brede O., Arnhold J. Formation of phosphatidic acid, ceramide, and diglyceride on radiolysis of lipids: identification by MALDI-TOF mass spectrometry. Free Radic. Biol. Med., 2004, 36(12), 1612-1624. DOI: 10.1016/j.freeradbiomed.2004.03.013
  5. Yurkova I., Kisel M., Arnhold J., Shadyro O. Iron-mediated free-radical formation of signaling lipids in a model system. Chem. Phys. Lipids., 2005, 137(1), 29-37. DOI: 10.1016/j.chemphyslip.2005.06.002
  6. L.L. Birichevskaya, L.A. Eroshevskaya, M.A. Kisel, A.I. Zinchenko. Substrate requirements of phospholipase D from Streptomyces netropsis in the transphosphatidylation synthesis of phospolipids. Chemistry of Natural Compounds, 2006, 42(1), 26-29. DOI: 10.1007/s10600-006-0030-9
  7. V.V. Bezuglov, N.M. Gretskaya, A.V. Blazhenova, E.L. Andrianova, M.G. Akimov, M.Yu. Bobrov, I.V. Nazimov, M.A. Kisel, O.L. Sharko, A.V. Novikov, N.V. Krasnov, V.P. Shevchenko, T.V. V'unova, N.F. Myasoedov. Arachidonoyl amino acids and arachidonoyl peptides: Synthesis and properties. Russian Journal of Bioorganic Chemistry, 2006, 32(3), 258-267. DOI: 10.1134/S1068162006030046
  8. Yurkova I.L., Stuckert F., Kisel M.A, Shadyro O.I., Arnhold J., Huster D. Formation of phosphatidic acid in stressed mitochondria. Arch. Biochem. Biophys., 2008, 480, 17-26. DOI: 10.1016/j.abb.2008.09.007
  9. Тростянко И.В., Долгопалец В.И., Кисель М.А. Методы получения 5-аминолевулиновой кислоты и ее липофильных производных. В сб. Биорегуляторы: исследование и применение. Под ред. Ф.А.Лахвича, ч.2, Минск, 2008, 154-164.
  10. Кисель М.А. Фосфолипиды и их роль в канцерогенезе / Липиды и рак. Очерки липидологии онкологического процесса. Под ред. В.В. Безуглова и С.С. Коновалова. – СПб.: Прайм-ЕВРОЗНАК, 2009, 161-193.
  11. M.V. Zhukova, O.V. Romanenko, V.A. Nikolaevich, M.A. Kisel. Hemolytic properties of miltefosine in liposomes of various lipid compositions. Pharmaceutical Chemistry Journal, 2010, 44(9), 29-31. DOI: 10.1007/s11094-010-0504-8
  12. Sharko O.L. Kisel M.A.1-Acyl-2-[N-(2,4-dinitrophenyl)aminopropionyl]-sn-glycero-3-phosphocholine as a chromogenic substrate for phospholipase A2 assay. Anal. Biochem., 2011, 413(1), 69-71. DOI: 10.1016/j.ab.2011.02.018

Collaboration with foreign and domestic research groups

  1. M.M. Shemyakin and Yu.A. Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences. Prof. Vladimir Bezuglov, D.Sc., Prof. George Shpakovsky, D.Sc.
  2. Belarusian State University. Prof. Oleg Shadyro, D.Sc.
  3. N.N. Alexandrov National Cancer Centre of Belarus. Prof. V.Prohorova, M.D., Prof. A.Mashevsky, M.D.
  4. Institute of Microbiology of the National Academy of Sciences of Belarus. Corresponding member of NASB, Prof. Anatoly Zinchenko, D.Sc.
  5. Institute of Biophysics and Cell Engineering of the National Academy of Sciences of Belarus. Elena Yaronskaya, D.Sc.

 

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